【摘 要】
：
Using microbeam to irradiate human-hamster hybrid AL cells withdefined number of a particles in a highly localized spatial region, this paper showed that cytopl
【机 构】
：
Institute of Plasma Physics,Center of Radiological Research
论文部分内容阅读
Using microbeam to irradiate human-hamster hybrid AL cells withdefined number of a particles in a highly localized spatial region, this paper showed that cytoplasmic irradiation induced very little toxicity. For example, the cell killing by 4 a particle traversal through the cytoplasm was about 10%, and about 70% cells survived after their cytoplasm was irradiated with 32 a particles. In contrast, the survival fractions for nuclear irradiation at the same doses were 35% and less than 1% respectively. Mutation induction showed that while nuclear irradiation induced 3-4-fold more CD59- mutants than cytoplasmic irradiation at equivalent particle traversal, at an equitoxic dose level of 90% survival, the latter exposure mode induced 3.3-fold more mutants than nuclear irradia-tion. Moreover, using multiplex PCR to analyze five marker genes on chromosome 11 (WT, CAT, PTH, APO-A1 and RAS), the results showed that the majority of mutants induced by cytoplasmic irradiation had retained all of the marker genes analyzed. By comparison, the proportion of mutants suffering loss of additional chromosomal markers increased with increasing number of particle traversal through nuclei.
其他文献
浙江省是工业锅炉使用大省,工业锅炉节能对浙江经济发展和节能减排具有举足轻重的作用。本文分析了浙江省工业锅炉耗能情况、结构、能效状况及节能潜力,归纳了存在主要问题,
An endogenous filariform fungus has been separated from a treenamed Taxus growing in the Aba region, Sichuan, China. The fungus is fermented in fluid medium for
本文通过对锅炉的整个运行过程的检验,针对各个环节进行分析,总结了工业锅炉在节能减排方面普遍存在的问题,在分析大量文献资料的基础上,结合实际工作经验,提出了一些节能减
Five overlapping clones covering the full genome of Enterovirus71 China strain SHZH98 were obtained and then the sequences were determined by the chain terminat
通过调查分析湘江“长株潭”段表层底泥的锌、铜、镉、铬、铅、汞、锰等重金属含量，将底泥样品中重金属的含量分别与土壤环境质量标准、污水排入城市下水道水质标准、以及生活
Using microfluorometry to assay intracellular Ca2+ , the influences of varied factors on glucoseinduced Ca22+ signals, such as glucose-induced initial decline p
Using a degenerated primer and a T-primer, a MADS-box gene, M79,was amplified by RT-PCR from rice fluorescence at meiosis stage and then cloned. Sequence analys
Upstream regulatory region and flanking DNA of yellow gene wereisolated and cloned from a Drosophila genomic library. A vector containing yellow gene and regula
Smad5 is an intracellular transducer of TGF-b signals. Targeteddisruption of murine Smad5 gene resulted in embryonic lethal. To study the function of Smad5 in o
The amber mutation sites of 6 purR(am) mutants were determined bycloning and DNA sequencing. The results showed that the mutations were distributed at three dif