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丹参是一种传统的中草药,对多种疾病有治疗作用,丹参粉针剂主要由其水溶性成分组成。在本项研究中,大鼠腹腔注射二甲基亚硝胺诱导肝纤维化,每周注射3天,连续4周。在造模2周后,阳性药组大鼠皮下注射8×105IU/kg IFNα2b;各治疗组分别腹腔注射50,100和200 mg/kg丹参粉针剂,每周给药6天,连续4周。结果表明,IFNα2b及3个治疗组的大鼠体重和肝脾比明显增加,而且3个治疗组大鼠的脾脏指数降低。血清检测结果显示,IFNα2b和丹参粉针剂可降低谷丙转氨酶,总胆红素,透明质酸,和Ⅲ型前胶原水平,其中100和200 mg/kg剂量组还明显改善了谷草转氨酶、白蛋白水平。在组织学检查中发现,IFNα2b和丹参粉针剂可改善肝细胞的状态,较少的纤维增生和胶原沉积。免疫组织化学染色显示,IFNα2b和丹参粉针剂显著下调了转化生长因子β1和血小板源性生长因子(PDGF)。总之,丹参粉针剂对二甲基亚硝胺导致的大鼠肝纤维化具有一定的保护作用,其机制可能与下调TGF-β1和PDGF有关。
Salvia is a traditional Chinese herbal medicine, a variety of diseases have a therapeutic effect, Salvia powder injection mainly composed of its water-soluble ingredients. In this study, rats were injected intraperitoneally with dimethylnitrosamine to induce liver fibrosis and were injected three days a week for 4 weeks. After 2 weeks of modeling, the rats in the positive group were subcutaneously injected with 8 × 105 IU / kg IFNα2b. Each treatment group was given intraperitoneal injection of 50, 100 and 200 mg / kg Salvia miltiorrhiza for 6 days a week for 4 weeks. The results showed that IFNα2b and three treatment groups of rats weight and liver-spleen ratio increased significantly, and the three treatment groups rats spleen index decreased. Serum test results showed that IFNα2b and Salvia powder injection can reduce the level of alanine aminotransferase, total bilirubin, hyaluronic acid, and type Ⅲ procollagen, of which 100 and 200 mg / kg dose group also significantly improved aspartate aminotransferase, albumin Level. Histological examination found that IFNα2b and Salvia powder injection can improve the state of liver cells, less fibrosis and collagen deposition. Immunohistochemical staining showed that IFNα2b and Salvia miltiorrhiza injection significantly down-regulated the expression of TGF-β1 and platelet-derived growth factor (PDGF). In conclusion, Danshen powder injection can protect rat liver fibrosis induced by dimethylnitrosamine, which may be related to the down-regulation of TGF-β1 and PDGF.