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目的观察依达拉奉对气道上皮细胞调亡及bcl-2mRNA、FADD的影响,探讨依达拉奉通过抗氧化机制减轻哮喘豚鼠气道上皮细胞的凋亡。方法 40只健康豚鼠随机分成4组:正常对照组(A)、哮喘组(B)、地塞米松治疗组(C)、依达拉奉治疗组(D),n=10,用卵清蛋白腹腔注射致敏及反复雾化刺激建立哮喘模型。原位杂交检测肺组织bcl-2mRNA表达,3’末端脱氧核苷转移酶介导的脱氧三磷酸尿苷(d-UTP)缺口末端标记(TUNEL)法检测细胞凋亡,采用生化、放免.免疫组化及形态学观察等方法。结果①B组MDA值明显高于D组与A组,SOD值明显低于D组与A组,D组与C组比较差异不显著。②D组BALF中IL-5含量与C组比无明显差别,明显低于B组。③FADD在B组表达增加,D组中表达降低。④D组气道上皮细胞凋亡减轻且与bcl-2mRNA、FADD有相关性。结论氧自由基清除剂依达拉奉能降低氧应激水平,上调bcl-2mRNA的表达,抑制FADD的表达,保护气道上皮细胞,减轻哮喘的反复发作。
Objective To observe the effect of edaravone on the apoptosis of airway epithelial cells and the expression of bcl-2mRNA and FADD, and to explore the mechanism of edaravone in reducing the apoptosis of asthmatic guinea pig airway epithelial cells through anti-oxidative mechanism. Methods Forty healthy guinea pigs were randomly divided into 4 groups: normal control group (A), asthma group (B), dexamethasone treatment group (C) and edaravone treatment group (D), n = Induction of intraperitoneal injection and repeated aerosol stimulation to establish asthma model. The expression of bcl-2 mRNA in lung tissue was detected by in situ hybridization and apoptosis was detected by 3’-terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL). The biochemical and radioimmunoassay Tissue and morphological observation and other methods. Results ① The MDA value of group B was significantly higher than that of group D and group A, the SOD value was significantly lower than that of group D and group A, and the difference between group D and group C was not significant. ② The level of IL-5 in BALF in D group was not significantly different from that in C group, which was significantly lower than that in B group. ③FADD expression increased in group B, D group decreased expression. ④ The apoptosis of airway epithelial cells in group D was alleviated and there was a correlation with bcl-2 mRNA and FADD. Conclusion The oxygen free radical scavenger edaravone can reduce the level of oxygen stress, up-regulate the expression of bcl-2 mRNA, inhibit the expression of FADD, protect the airway epithelial cells and reduce the recurrent asthma.