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目的研究核因子κB受体活化因子配基(RANKL)在人继承恒牙缺失滞留乳牙及乳牙根生理性吸收不同时期的表达。方法选取2010年6-12月沈阳市口腔医院正畸科及儿童牙病科10～15岁患者因治疗需要拔除的乳牙18颗,按牙根吸收长度不同分为牙根吸收早、中、晚期(早期组、中期组、晚期组),各6颗。同时选取无恒牙胚滞留乳牙(滞留组)和正畸拔除的正常恒牙(对照组)各6颗。采用免疫组化方法检测RANKL蛋白的表达,并测定累积光密度值。结果牙髓成纤维细胞:对照组RANKL累积光密度值明显低于其余组(均P<0.01);早期组、中期组明显低于晚期组(均P<0.01)。成牙本质细胞:对照组RANKL累积光密度值亦明显低于其余组(均P<0.01);早期组、中期组、晚期组三者间差异均有统计学意义(P<0.01)。破牙细胞:对照组未见破牙细胞;早期组、中期组与晚期组比较差异有统计学意义(P<0.01)。结论 RANKL是引起乳牙牙根缓慢吸收的因素之一。
Objective To study the expression of RANKL at different stages of human physiological inheritance of deciduous teeth and deciduous teeth root. Methods 18 cases of deciduous teeth were collected from Department of Orthodontics and Pediatric Dentistry of Shenyang Stomatology Hospital from January to December in 2010. According to the length of root resorption, the teeth were divided into early, middle and late stages (early stage Group, mid-term group, late group), each 6. At the same time, 6 permanent teeth (control group) without permanent tooth germ retention deciduous teeth (retention group) and normal permanent teeth (control group) were selected. The expression of RANKL protein was detected by immunohistochemistry and the cumulative optical density was measured. Results Pulmonary fibroblasts: The optical density of RANKL in the control group was significantly lower than that in the other groups (all P <0.01). The early and middle groups were significantly lower than those in the advanced group (all P <0.01). Odontoblasts: The cumulative optical density of RANKL in the control group was also significantly lower than the other groups (all P <0.01). There were significant differences among the three groups in early stage, middle stage and late stage (P <0.01). Broken tooth cells: There were no broken cells in control group. There were significant differences between early group, middle group and late group (P <0.01). Conclusion RANKL is one of the factors that cause the slow absorption of root of deciduous teeth.