目的 :EAN中神经抗原 (P2蛋白 )的有效表位的鉴定以及将其与空肠弯曲菌 (Campylobacterjejuni,Cj)LPS抗原性比较。方法 :采用噬菌体呈现技术对 8个EAN模型血清抗体进行肽库筛选 ,并将获得的克隆用ELISA方法进行阳性鉴定 ,以及将阳性克隆与空肠弯曲菌LPS相似性进行比较。结果 :33个阳性克隆的亲和性均显著高于阴性对照 (P <0 0 1) ,说明它们是具有特异性的克隆 ;C J LPS与血清的亲和力显著高于对照组 (P <0 0 1) ,与阳性克隆的OD值相差不显著 ,C J LPS与阳性克隆之间存在相似性。结论 :噬菌体呈现技术可筛选出与P2蛋白具有相同表位的噬菌体克隆 ;空肠弯曲菌LPS与P2蛋白之间存在表位模拟 ,C J LPS可能是启动GBS自身反应性免疫应答的重要因素。 OBJECTIVE: To identify effective epitopes of neuronal antigens (P2 proteins) in EAN and to compare them against the antigenicity of Campylobacter jejuni (Cj) LPS. Methods: The phage display technique was used to screen the serum of eight EAN models. The positive clones were identified by ELISA. The positive clones were compared with the LPS of Campylobacter jejuni. Results: The positive rates of 33 positive clones were significantly higher than that of the negative control (P <0.01), indicating that they were specific clones. The affinity of CJ LPS with serum was significantly higher than that of the control group (P <0.01 ), And OD value of the positive clone was not significantly different, there is a similarity between CJ LPS and positive clones. Conclusion: The phage display technique can screen out phage clones with the same epitope as P2 protein. There is epitope simulation between Campylobacter jejuni LPS and P2 protein. C J LPS may play an important role in initiating GBS autoreactive immune response.