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用两种方法进行了兰花"OtochilusalbaLindL."的微型繁殖。1.以MS培养基为基本培养基,附加0.5mg/L(单位下同)NAA和2.0Lip;2.利用Phytamax兰花繁殖培养基,使从假鳞茎基部产生的匍匐茎进一步发育,在匍匐茎的末端形成鳞茎结构最终形成10~15个带两个枝叶的假鳞茎。包括母体鳞茎在内的所在假鳞茎繁殖迅速形成了25~35个假鳞茎。最后,在一个培养容器中50天内形成了100多个假鳞茎。单个假鳞茎在IAA或IBA存在下生了根。
Micropropagation of orchids “Otochilusalba Lindl.” Was performed in two ways. 1. With MS medium as the basic medium, add 0.5mg / L (unit the same below) NAA and 2.0Lip; The stolons produced from the base of the pseudobulb were further developed using Phytamax orchid propagation medium, forming bulb structures at the ends of stolons eventually forming 10-15 pseudobulb with two foliage. The pseudobulbs, including maternal bulbs, formed rapidly from 25 to 35 pseudobulbs. Finally, more than 100 pseudobulses were formed in 50 days in a culture vessel. A single pseudobulb had roots in the presence of IAA or IBA.