论文部分内容阅读
以前实验表明,可溶性β-1,3葡聚糖(简称SG)对多种实验性肿瘤、病原微生物感染及免疫反应低下有效.鉴于SG的免疫调节作用,本文应用C57BL/6J小鼠胸腺细胞及CTLL-2细胞(IL-2依赖细胞株)增殖法分别对Sprague-Dawley大鼠在接受SG(100mg/kg,ip)后不同时间(1h～9d)脾脏巨噬细胞产生IL-1和脾脏淋巴细胞产生IL-2的作用以及血浆1L-1和IL-2水平进行评价,同时对不同给药途径(100mg/kg,sc,iP,iv)对脾脏巨噬细胞产生IL-1及脾淋巴细胞生产IL-2的效能进行比较.结果表明,给SG(ip)后12h,脾巨噬细胞产生IL-1量开始明显升高,持续5天;SG对脾细胞分泌IL-2的增强作用始于给药后6h,3～9天达高峰,持续升高达12天.给SG
Previous experiments showed that soluble β-1,3-glucan (SG) is effective for a variety of experimental tumors, pathogenic microorganisms and immune response.Considering the immunomodulatory effects of SG, we used C57BL / 6J mouse thymocytes and Proliferation of CTLL-2 cells (IL-2-dependent cell lines) Spleen-Dawley rats were treated with SG (100mg / kg, ip) at different times (1h ~ 9d) spleen macrophages produce IL- IL-2 production and plasma 1L-1 and IL-2 levels were evaluated. At the same time, IL-1 and splenic lymphocytes were produced from splenic macrophages by different route of administration (100mg / kg, sc, The results showed that the amount of IL-1 produced by splenic macrophages began to increase significantly at 12h after SG (ip) administration, which lasted for 5 days. SG enhanced the secretion of IL-2 from spleen cells 6h after administration, 3 to 9 days peaked, continued to rise for up to 12 days to SG