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目的观察变频振动在模拟微重力环境下对体外培养成骨细胞增殖和分化的影响。方法利用沿水平轴连续回转(30 r/min)细胞培养系统模拟微重力环境,应用电磁振动台将振动强度为0.5 g(g为加速度)在不同频率45 Hz、90 Hz、变频(5~90 Hz)振动应力作用于新生24 h大鼠颅盖骨成骨细胞,分别使用MTT比色法和碱性磷酸酶(ALP)活性测定了解成骨细胞的增殖和分化情况。结果模拟微重力环境可以抑制新生24 h大鼠颅盖骨成骨细胞的增殖和分化功能;90 Hz和5~90Hz变频振动促进模拟微重力环境成骨细胞的增殖功能(p<0.01);45 Hz(p<0.05)和5~90 Hz(p<0.01)变频振动刺激对模拟微重力环境成骨细胞的分化功能具有一定的保护作用。结论在模拟微重力环境下机械振动对成骨细胞的增殖和分化功能具有保护作用,这为机械振动刺激防治微重力环境下骨丢失提供了理论和实验依据。
Objective To observe the effects of frequency-varying vibration on the proliferation and differentiation of cultured osteoblasts under simulated microgravity. Methods The microgravity environment was simulated by a continuous rotation (30 r / min) cell culture system along the horizontal axis. The vibration amplitude was 0.5 g (g is acceleration) at different frequencies of 45 Hz, 90 Hz, Hz) vibrational stress on calvaria osteoblasts of neonatal rats for 24 h. The proliferation and differentiation of osteoblasts were determined by MTT assay and alkaline phosphatase (ALP) activity respectively. Results Simulated micro-gravity environment could inhibit the proliferation and differentiation of calvaria osteoblasts in 24 h neonatal rats. The frequency-doubling vibration of 90 Hz and 5-90 Hz promoted the proliferation of osteoblasts in simulated microgravity environment (p <0.01). Hz (p <0.05) and 5 ~ 90 Hz (p <0.01). VVF stimulation may play a protective role in the differentiation of osteoblasts under simulated microgravity. Conclusions Mechanical vibration plays a protective role in the proliferation and differentiation of osteoblasts under simulated microgravity. This provides a theoretical and experimental basis for the prevention of bone loss under microgravity by mechanical vibration stimulation.