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目的:探讨白藜芦醇(Resveratrol,Res)对人神经母细胞瘤SH-SY5Y增殖变化规律和生长状况的影响。方法:通过不同浓度(0.15～500)μmol/LRes作用于SH-SY5Y,检测药物作用5d后各组细胞增殖率和细胞生长状态,同时通过增殖率曲线获得其半数生长抑制浓度(IC50)和杀伤浓度,并对各组细胞进行Hochest染色,检测其细胞凋亡情况。结果:Res5μmol/L组细胞增殖率为(117.00±15.30)%显著高于空白对照组(P<0.05),15μmol/L组细胞增殖率为(31.33±2.89)%,50、150、500μmol/L组,细胞出现负增长。Res对SH-SY5Y的IC50为12.78μmol/L,细胞杀伤浓度(即增殖率为0%)为19.88μmol/L。Res50～500μmol/L各组内,部分细胞皱缩,突起减少或消失,并出现凋亡细胞。结论:Res在0.15～500μmol/L浓度范围内对SH-SY5Y的增殖影响存在浓度依赖性,并依次表现为促进增殖、抑制增殖和杀伤细胞三个方面效应。其中杀伤细胞效应与促进SH-SY5Y的凋亡启动相关。
Objective: To investigate the effects of resveratrol (Res) on the proliferation and proliferation of human neuroblastoma SH-SY5Y. METHODS: SH-SY5Y cells were treated with various concentrations (0.15-500) μmol / L Res for 5 days. The cell proliferation rate and cell growth status of all groups were also measured. The IC50 values and cytotoxicity The cells were stained with Hochest and the cell apoptosis was detected. Results: The cell proliferation rate was (117.00 ± 15.30)% in Res5μmol / L group was significantly higher than that in blank control group (31.33 ± 2.89)%, 50,150,500μmol / L Group, cells showed negative growth. Res for SH-SY5Y IC50 of 12.78μmol / L, cell killing concentration (ie, the proliferation rate of 0%) was 19.88μmol / L. Res 50 ~ 500μmol / L groups, part of the cells shrinkage, protrusion decreased or disappeared, and apoptotic cells. CONCLUSION: Res concentration in the range of 0.15-500 μmol / L has a concentration-dependent effect on the proliferation of SH-SY5Y, which in turn has the effect of promoting proliferation, inhibiting proliferation and killing the cells. The killing effect was related to the initiation of apoptosis of SH-SY5Y.