Acacia senegal, the gum arabic producing tree, is the most important tree species for the livelihood of the people in South Kordofan State, Sudan. The objective of this study was to determine the optimum tapping date for gum arabic production in the study area. A randomized complete block design experiment with three replications was conducted at (Meari) area for two continuous growing seasons 2008/2009 - 2009/2010. The treatments comprised six tapping dates (1 Oct, 15 Oct, 1 Nov, 15 Nov, 1 Dec, and 15 Dec). Results showed highly significant differences (p < 0.001) in gum arabic yield (gtree) in all pickings and in the total gum yield between the tapping dates. The results also showed that tapping of trees on 15 October and 1 November gave a higher yield compared to the other dates. The highest gum yield of 1086.6 and 661.2 g/tree was recorded on 15 October and 15 November, while the lowest gum yield of 297.9 g/tree was recorded when the trees were tapped on 1 October. The two highest-yield dates of tapping (15 Oct and 1 Nov) are recommended as the best time for tapping for gum arabic production in South Kordofan State. These results can be used to increase gum arabic production and farmer income in South Kordofan State. Acacia senegal, the gum arabic producing tree, is the most important tree species for the livelihood of the people in South Kordofan State, Sudan. The objective of this study was to determine the optimum tapping date for gum arabic production in the study area. A randomized complete block design experiment with three replications was conducted at (Meari) area for two continuous growing seasons 2008/2009 - 2009/2010. The protocols became six tapping dates (1 Oct, 15 Oct, 1 Nov, 15 Nov, 1 Dec, and 15 Dec). Results showed highly significant differences (p <0.001) in gum arabic yield (g tree) in all pickings and in the total gum yield between the tapping dates. The results also showed that tapping of trees on 15 October and 1 November gave a higher yield compared to the other dates. The highest gum yield of 1086.6 and 661.2 g / tree was recorded on 15 October and 15 November, while the lowest gum yield of 297.9 g / tree was recorded when the trees were tapped on 1 October. The two highes t-yield dates of tapping (15 Oct and 1 Nov) are recommended as the best time for tapping for gum arabic production in South Kordofan State. These results can be used to increase gum arabic production and farmer income in South Kordofan State.