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利用SSR分子标记技术,对采自中国新疆的51份野生狗牙根(Cynodon dactylon)及19份新疆农业大学选育的材料进行遗传多样性研究。结果表明,11对引物共得到多态性条带数55条,平均每对引物扩增出5条带,每对引物检测到3~6个等位基因,平均每对引物检测到5个等位基因。每个SSR位点的多态性信息量(PIC)在0.65~0.83,平均0.78;SSR标记揭示的新疆70份材料间的遗传相似系数变化范围为0.50~0.84,表明新疆狗牙根具有较丰富的遗传多样性。UPGMA聚类分析表明,SSR标记能够将新疆70份材料区分开;供试材料可聚为4类,其中Cd071单独聚成1类,野生材料聚为2类。表明这些材料遗传差异较大,各生态地理类群间的遗传分化与其所处的生态地理环境具有一定的相关性。
Using SSR markers, genetic diversity was studied in 51 wild Cynodon dactylon collected from Xinjiang, China and 19 from Xinjiang Agricultural University. The results showed that a total of 55 polymorphic bands were obtained with 11 pairs of primers, with an average of 5 bands for each pair of primers, 3 to 6 alleles for each pair of primers, 5 pairs for each pair Bit gene. The PIC of each SSR loci ranged from 0.65 to 0.83 with an average of 0.78. The genetic similarity coefficient revealed by SSR markers ranged from 0.50 to 0.84 in 70 germplasm resources in Xinjiang, suggesting that Xinjiang Bermudagrass has a richer Genetic diversity. UPGMA cluster analysis showed that SSR markers could distinguish 70 materials in Xinjiang. The tested materials could be classified into 4 types, of which Cd071 was aggregated into 1 type and wild type was classified into 2 types. The results showed that there was a great genetic difference among these materials, and the genetic differentiation among ecophysiological groups was related to the ecological environment in which they lived.