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目的探索鼠模型动静脉畸形在放射外科治疗后血清中炎症分子的改变,为进一步寻找人类脑动静脉畸形对放射外科治疗反应的早期监测标志物奠定基础。方法将12只雄性SD大鼠随机分成实验组和对照组,每组6只。利用显微血管吻合法进行造模,造模后42 d,利用酶联免疫吸附试验分别定量测定两组大鼠放疗前血清中可溶性E-选择素、P-选择素、细胞间黏附分子-1(SICAM-1)、血管细胞黏附分子-1(SVCAM-1)、组织因子及实验组放疗后6、12、24、48、72、120 h血清中以上各因子的含量,并利用SPSS17.0统计学软件进行数据分析。结果放疗后实验组大鼠血清可溶性E-选择素及P-选择择素、SICAM-1、SVCAM-1及组织因子较放疗前及对照组升高。实验组放疗不同时间点各种炎症分子的表达有差异:放疗后6、12 h时,实验组血清中可溶性E-选择素的平均浓度较放疗前显著升高(P<0.05);放疗后6、12、24、48、72 h时血清中可溶性P-选择素的水平与放疗前比较显著升高(P<0.05);放疗后6 h和12 h时,血清中sICAM-1和sVCAM-1的浓度较放疗前均显著升高(P<0.05);放疗后6 h血浆可溶性组织因子浓度较放疗前升高1倍,在放疗后24 h内维持在高水平(P<0.05)。结论在放射外科治疗动静脉畸形的过程中在不同时间点对以上炎症分子进行组合监测可作为早期预测动静脉畸形对于放射外科治疗反应的标志物。
Objective To explore the changes of inflammatory molecules in rat model of arteriovenous malformation after radiosurgery treatment and lay the foundation for the further search of human cerebral arteriovenous malformations for early monitoring of radiosurgery response. Methods Twelve male SD rats were randomly divided into experimental group and control group, with 6 rats in each group. The microvascular anastomosis was used to make model. The levels of soluble E-selectin, P-selectin and intercellular adhesion molecule-1 were determined by enzyme-linked immunosorbent assay (ELISA) (SICAM-1), vascular cell adhesion molecule-1 (SVCAM-1), tissue factor and the experimental group 6,12,24,48,72,120 h after the treatment of serum levels of the above factors, and using SPSS17.0 Statistical software for data analysis. Results Serum levels of soluble E-selectin and P-selectin, SICAM-1, SVCAM-1 and tissue factor in experimental group were significantly higher than those before radiotherapy and control group after radiotherapy. At 6 and 12 h after radiotherapy, the mean concentration of soluble E-selectin in the experimental group was significantly higher than that before radiotherapy (P <0.05); after radiotherapy, the expression of various inflammatory molecules in the experimental group was different at different time points after radiotherapy Serum levels of soluble P-selectin at 12, 24, 48 and 72 h were significantly higher than those before radiotherapy (P <0.05); at 6 and 12 h after radiotherapy, serum sICAM-1 and sVCAM-1 (P <0.05). At 6 hours after radiotherapy, the concentration of soluble tissue factor in plasma increased by 1 time compared with that before radiotherapy and maintained at a high level within 24 hours after radiotherapy (P <0.05). Conclusion Combined monitoring of these inflammatory molecules at different time points in the treatment of arteriovenous malformations during radiosurgery can be used as a marker for the early prediction of arteriovenous malformation response to radiosurgery.