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以多糖与苯酚-浓硫酸缩合成有色有机物在可见光区490.0 nm处有最大吸收为依据,以葡萄糖为标样,采用分光光度法测定了绣线菊根中多糖的含量,建立了葡萄糖标准工作曲线的回归方程,线性相关系数R=0.9996.通过正交试验法对温度、次数、时间及料液比等因素对提取率的影响进行了研究.绣线菊根中多糖的最佳提取工艺条件是用20倍水量在90℃温度下回流提取3次,每次水提取时间为3 h.该条件下测量的欧亚绣线菊根中多糖的含量为6.3068 mg/g,精密度试验中RSD=1.03%,稳定性试验中RSD=1.19%,加标回收率平均值为97.45%,RSD=1.79%.该方法操作简单易行,结果稳定可靠,而提取多糖过程中使用的溶剂水对植物组织的穿透力强并且环保.
Polysaccharides and phenol - concentrated sulfuric acid condensation into colored organic matter in the visible light 490.0 nm maximum absorption as the basis, with glucose as the standard sample, the polysaccharide content of Spiraea root was determined by spectrophotometry, the establishment of a glucose standard working curve , The linear correlation coefficient R = 0.9996.The effects of temperature, times, time and the ratio of solid to liquid on the extraction rate were studied by orthogonal test.The optimal extraction conditions of polysaccharides from Spiraea is 20 times the amount of water at 90 ℃ under refluxing extraction three times, each time the water extraction time was 3 h under the conditions of the polysaccharide content of Eucommia Spiraea root 6.3068 mg / g, precision test RSD = 1.03%, stability test RSD = 1.19%, the average recovery of the standard was 97.45%, RSD = 1.79%. The method is simple and easy to operate, the results are stable and reliable, and the extraction of polysaccharides used in the process of solvent water on plant tissue The penetration and environmental protection.