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目的:从血管生成素(Ang)及酪氨酸激酶受体(Tie)表达方面揭示脑脉通促进老龄大鼠脑缺血/再灌注血管生成的作用机制。方法:将SD雄性老龄大鼠随机分为假手术组、模型组、尼莫地平组、脑脉通组,运用免疫组化和原位杂交等方法测定Ang-1、Ang-2,及其受体Tie-2的蛋白与基因表达。结果:脑脉通组各时间点Ang-1、Ang-2、Tie-2蛋白表达均较模型组增强(P<0.05,P<0.01)。脑脉通组缺血3h及脑缺血/再灌注3d、6d、12dAng-2 mRNA以及各时间点Ang-1 mRNA、Tie-2 mRNA表达水平均较模型组增强(P<0.01)。结论:脑脉通促进老年脑缺血/再灌注微血管生成的机制可能与提高Ang/Tie系统蛋白和基因表达有关。
OBJECTIVE: To explore the mechanism of Naomaitong’s promoting angiogenesis in aged rats with cerebral ischemia / reperfusion based on the expression of angiopoietin (Ang) and tyrosine kinase receptor (Tie). Methods: SD male aged rats were randomly divided into sham-operation group, model group, nimodipine group and Naomaitong group. Ang-1, Ang-2 and its receptor were detected by immunohistochemistry and in situ hybridization Body Tie-2 protein and gene expression. Results: The expression of Ang-1, Ang-2 and Tie-2 in Naomaitong group were higher than those in model group at each time point (P <0.05, P <0.01). The Ang-1 mRNA and Tie-2 mRNA expression in the Naomaitong group for 3h, 3h, 6d, 12d after cerebral ischemia / reperfusion were significantly higher than those in the untreated group (P <0.01). Conclusion: The mechanism of Naomaitong promoting cerebral ischemia / reperfusion microenvironment may be related to the increase of Ang / Tie system protein and gene expression.