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目的研究p53、p27和mdm2蛋白在胃肠道间质瘤(GIST)中的表达及其与GIST生物学行为的关系,为预测该肿瘤的侵袭潜能提供理论依据和可能的预测指标。方法收集68例手术切除的GIST标本,应用免疫组织化学法检测肿瘤组织中p53、p27和mdm2蛋白的表达,并结合病理形态学及生物学行为评价进行统计学分析。结果68例GIST中p53、p27和mdm2蛋白阳性表达率分别为75%、54.41%和27.94%。p53阳性表达率在极低度侵袭危险性(VLR)、低度侵袭危险性(LR)和高度侵袭危险性(HR)组三组间,细胞核分裂数≤10/50 HPF和>10/50 HPF两组间的差别均有统计学意义(P<0.05),在肿瘤直径≤5 cm和>5 cm两组间差异有高度统计学意义(P<0.01);p27阳性表达率在VLR、LR和HR组间比较有统计学意义(P<0.05),在肿瘤直径≤5 cm和>5 cm两组间差别有高度统计学意义(P<0.01);mdm2阳性表达率在细胞核分裂数≤10/50HPF和>10/50HPF两组间比较差别有统计学意义(P<0.05)。结论p53、p27和mdm2蛋白表达与GIST的发生、发展有关,检测p53、p27及mdm2表达可以作为GIST生物学行为评价的有效指标。
Objective To study the expression of p53, p27 and mdm2 protein in gastrointestinal stromal tumors (GIST) and its relationship with the biological behavior of GIST, and to provide theoretical basis and possible prognostic indicators for predicting the invasion potential of this tumor. Methods Sixty-eight surgical specimens of GIST were collected. The expressions of p53, p27 and mdm2 protein in tumor tissue were detected by immunohistochemistry. Statistical analysis was made according to pathomorphology and biological behavior evaluation. Results The positive rates of p53, p27 and mdm2 in 68 cases of GIST were 75%, 54.41% and 27.94%, respectively. The positive rates of p53 were between the three groups of VLR, LR and HR, and the numbers of mitosis were less than 10/50 HPF and> 10/50 HPF The difference between the two groups was statistically significant (P <0.05), and there was a significant difference between the two groups (P <0.01). The positive rate of p27 was significantly higher in VLR, LR and The difference between the two groups was statistically significant (P <0.05), but there was a significant difference between the two groups (P <0.01). The positive rate of mdm2 was higher 50HPF and> 10 / 50HPF difference between the two groups was statistically significant (P <0.05). Conclusion The expressions of p53, p27 and mdm2 proteins are related to the occurrence and development of GIST. Detecting the expression of p53, p27 and mdm2 may be useful indexes for evaluating the biological behavior of GIST.