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以扁桃(Amygdalus conmmuis L.)幼叶为试材,通过7种保存方法并采用改良2×CTAB法、3×CTAB法和SDS法对其DNA进行提取,用琼脂糖凝胶电泳检测其DNA完整性,分光光度计测其产量和纯度,以探讨不同保存方法和不同提取方法对扁桃DNA提取质量的影响。结果表明,扁桃新鲜幼叶、压干幼叶后在-75℃保存、45℃烘干幼叶后在-75℃保存以及硅胶干燥幼叶后在-75℃保存三个月均可以用三种提取方法可获得完整的DNA,纯度和产量均高,其保存和提取方法简便易行且获得高质量DNA。扁桃鲜叶直接放在-20℃保存比-75℃保存更易获得DNA,而压干后的扁桃叶片-20℃保存三个月并采用SDS法提取,获得高产量、高纯度的DNA,不影响提取效果。SDS法和改良3×CTAB法均可获得扁桃DNA,但SDS法优于3×CTAB法,其操作简单、耗时少;改良2×CTAB法提取扁桃成功率较低,不稳定,产量比其它两种方法低。
The young leaves of Amygdalus conmmuis L. were used as materials, and the DNAs were extracted by seven kinds of preservation methods and improved by 2 × CTAB method, 3 × CTAB method and SDS method. The DNA was detected by agarose gel electrophoresis Sex, spectrophotometer to measure the yield and purity, in order to explore the different preservation methods and different extraction methods on Almond quality of DNA extraction. The results showed that the fresh young leaves of almond could be stored at -75 ℃ after the young leaves were dried, stored at -75 ℃ after drying at 45 ℃, and kept at -75 ℃ for three months after the young leaves were dried. The extraction method can obtain complete DNA with high purity and high yield, and the method for storing and extracting the same is simple and convenient and obtains high-quality DNA. Fresh almond leaves were stored directly at -20 ° C and stored at -75 ° C for easier access to DNA, whereas pressed-dried almond leaves were stored at -20 ° C for three months and extracted by SDS to obtain high-yield, high-purity DNA without affecting Extract the effect. SDS method and modified 3 × CTAB method can obtain almond DNA, but SDS method is better than 3 × CTAB method, which is simple and time-consuming; Improved 2 × CTAB method of almond extraction success rate is low, unstable, yield than other The two methods are low.