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目的:了解广东省食品中O157:H7大肠杆菌分离株中生化特征、毒力因子的携带与耐药情况。方法:采用免疫磁珠富集法进行O157:H7大肠杆菌分离,对O157:H7大肠杆菌分离株进行生化、噬菌体、血清学鉴定后,应用PCR技术检测其毒力因子,应用纸片法(K-B法)进行药敏试验。结果:从277份样品中分离出2株O157:H7大肠杆菌,总检出率为0.72%;对这2株O157:H7大肠杆菌分离株进行PCR毒力因子的测定,其中1株为携带eaeA、H ly和SLT2毒力因子的强毒株,而另1株分离株未检出毒力因子。药敏试验结果显示,这两个O157:H7大肠杆菌分离株对青霉素类、四环素类、磺胺类、喹诺酮类、大环内酯类5大类的部分抗生素有多重耐药性。结论:广东省食品中存在O157:H7大肠杆菌强毒株的污染。O157:H7大肠杆菌多重耐药株的出现,提示应关注我国畜牧养殖业抗生素的使用情况。
Objective: To understand the biochemical characteristics and virulence factors of O157: H7 Escherichia coli isolates in Guangdong Province. Methods: Immunomagnetic enrichment was used to isolate O157: H7 Escherichia coli. After biochemical, phage and serological identification of the O157: H7 Escherichia coli isolates, virulence factors were detected by PCR. France) for drug susceptibility testing. Results: Two O157: H7 Escherichia coli isolates were isolated from 277 samples with a total detection rate of 0.72%. PCR was used to determine the virulence factors of these two strains of E. coli O157: H7. Among them, one strain harboring eaeA , H ly and SLT2 virulence factor virulence strains, while the other isolates did not detect virulence factors. The result of drug sensitivity test showed that the two O157: H7 Escherichia coli isolates were multi-resistant to some antibiotics such as penicillins, tetracyclines, sulfonamides, quinolones and macrolides. Conclusion: The contamination of O157: H7 Escherichia coli strain in food of Guangdong Province exists. The emergence of O157: H7 Escherichia coli multi-drug resistant strains suggests that we should pay attention to the use of antibiotics in livestock husbandry in our country.