体液中药物及其代谢产物含量极微,成分复杂,且常与蛋白质或其它大分子结合;所涉及的问题又不仅是单一组分的分析,更多的是需同时测定多种组分的含量,要求其分析方法灵敏度高和专一性强。色谱和光谱技术的应用,特别是色谱与光谱联用技术的迅速发展,已为解决这一课题提供了有力的手段。近年来应用色谱技术分析体液中药物及其代谢产物的方法报道甚多,Gudzinowicz等详细综述了一些药物及其代谢产物用气相色谱法或联用气相色谱-质谱定性、定量分 Body fluids and their metabolites in trace amounts, complex composition, and often with protein or other macromolecules; The problems involved not only the analysis of a single component, more is the need to simultaneously determine the content of multiple components , Requiring its analytical method of high sensitivity and specificity. The application of chromatography and spectroscopy, especially the rapid development of chromatography and spectroscopy technology, has provided a powerful solution to this problem. In recent years, the application of chromatographic techniques for the analysis of body fluids and their metabolites has been reported in many ways. Gudzinowicz et al. Reviewed in detail the methods for the qualitative and quantitative analysis of some drugs and their metabolites by gas chromatography or gas chromatography-mass spectrometry