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目的 :比较由不同实验者制备的棉球及固定大小的Schirmer试验用滤纸片中所含丝裂霉素的重量。方法 :三位实验者按照要求分别制备 2 0个棉球 ( 4mm× 5mm) ,同时使用同一个打孔器分别制备 2 0个圆形滤纸片 (直径为 6mm) ,每一个棉球和滤纸片分别浸入浓度为 0 4mg/ml的丝裂霉素溶液中 5秒钟 ,于浸入之前后测量每一个棉球和滤纸片的干重和湿重。结果 :棉球中所含丝裂霉素的平均重量为 2 6 3± 6 8μg ,不同实验者间差异有显著性 (P <0 0 0 0 1 ) ;Schirmer试验用滤纸片中所含丝裂霉素的平均重量为 4 7± 0 1 μg ,不同实验者间差异无显著性 (P =0 0 558)。结论 :目前临床上应用的棉球携载丝裂霉素的方法 ,因术者不同其所含丝裂霉素的重量差异很大 ,药物的实际用量很难控制 ,而应用Schirmer试验滤纸片携载丝裂霉素的方法具有较好的可重复性 ,药物的实际用量较易控制 ,具有在临床中应用的前景。
OBJECTIVE: To compare the weight of mitomycin contained in cotton balls prepared by different experimenters and Schirmer test paper sheets of fixed size. Methods: Three experimenters prepared 20 cotton balls (4mm × 5mm) respectively according to the requirements. At the same time, 20 circular filter pieces (6mm in diameter) were prepared respectively by the same punch. Each cotton ball and filter paper Were immersed in a solution of mitomycin at a concentration of 0 4 mg / ml for 5 seconds, and the dry weight and wet weight of each cotton ball and filter paper were measured before and after immersion. Results: The average weight of mitomycin contained in cotton balls was 26 ± 6 8 μg, with significant difference (P <0 0 001) between different experimental subjects. The mitochondria contained in the Schirmer test The mean weight of mycotoxin was 47 ± 0 1 μg. There was no significant difference among different groups (P = 0 0 558). Conclusion: At present, the clinical application of cotton ball carrying mitomycin method, due to different surgeons contained a great difference in the weight of mitomycin, the actual dosage of the drug is difficult to control, but the use of Schirmer test filter paper to carry Mitomycin containing mitomycin method has good reproducibility, the actual dosage of the drug easier to control, with the clinical application prospects.