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尖镰孢寄主范围广、遗传差异大,其种下存在多种寄主专化型。对尖镰孢寄主专化型的快速鉴定可为科学制定植物病害防控策略提供依据。利用Sherlock MIS脂肪酸鉴定系统对分离自番茄、棉花、黄瓜、茄子等4种寄主专化型的18株尖镰孢进行脂肪酸成分测定,共检测到10种脂肪酸。运用SPSS软件中的PCA法对被检测到的脂肪酸进行主成分分析,确定了18:1CIS9(W9)[X1],18:2 CIS 9,12/18:0a[X2]和18:00[X3]等3个脂肪酸为其主成分。利用Bayes逐步判别法建立了尖镰孢4种不同寄主专化型判别模型为Y1=‐157.750+2.809X1+3.391X2+8.099X3;Y2=‐178.343+0.586X1+7.587X2‐0.214X3;Y3=‐129.132+2.749X1+4.163X2+4.476X3;Y4=‐201.307+2.016X1+7.345X2+2.400X3。通过对43株未知寄主专化型菌株主成分脂肪酸的测定,利用判别法对尖镰孢进行判定,结果发现有40株与原寄主来源一致,判对率达93%。表明脂肪酸生物标记法可用于尖镰孢寄主专化型的快速鉴定。
Fusarium oxysporum host a wide range of genetic differences, there are many species of its host specialized. The rapid identification of Fusarium oxysporum f. Sp. Predominately can provide scientific basis for plant disease prevention and control strategies. The fatty acids of 18 Fusarium oxysporum f. Sp. Isolates from four host-specific isolates from tomato, cotton, cucumber and eggplant were assayed by Sherlock MIS fatty acid assay system. Ten fatty acids were detected. Principal component analysis (PCA) of the detected fatty acids was performed using the PCA method of SPSS software, and 18: 1CIS9 (W9) [X1], 18: 2 CIS9,12 / 18: 0a [X2] and 18:00 [X3 ] And other three fatty acids as its main component. The discriminant model of Fusarium oxysporum Fusarium oxysporum was established by using Bayesian stepwise discriminant method. Y1 = -157.750 + 2.809X1 + 3.391X2 + 8.099X3; Y2 = -178.343 + 0.586X1 + 7.587X2-0.214X3; Y3 = -129.132 + 2.749X1 + 4.163X2 + 4.476X3; Y4 = -201.307 + 2.016X1 + 7.345X2 + 2.400X3. Fusarium oxysporum fusiformis was determined by the method of determination of 43 fatty acids, which is the main component of unknown strains of host-specific strains. The results showed that 40 strains were identical with the original host, and the correctness rate was 93%. It shows that the fatty acid biomarker method can be used for the rapid identification of Fusarium oxysporum f.