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目的分析排铅食品影响铅中毒小鼠全血、脑组织中微量元素及抗氧化酶的剂量效应关系。方法选用75只18～22 g昆明种雄性小鼠,随机分为阴性对照组、醋酸铅模型组和排铅食品低、中、高剂量组。除阴性对照组外,其他组均自由饮用1.00 g.L-1醋酸铅水溶液染毒3,0 d后,排铅食品组按人体推荐摄入量0.60g.kg-1.d-1的51、0和20倍经口灌胃;阴性对照组和醋酸铅模型组灌胃去离子水。1个月后测全血、脑组织中微量元素(Ca、Pb、Fe、Cu、Zn)以及超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)和丙二醛(MDA)的含量。结果铅中毒小鼠全血和脑组织中Pb、Ca、Cu以及MDA含量随着排铅食品剂量的增加而降低,Fe、Zn含量、SOD和GSH-PX活性随着排铅产品剂量的增加而增加。结论该排铅食品在排铅的同时,可有效调节全血和脑组织微量元素的平衡,增加抗氧化物酶活性,降低脂质过氧化物的产生。
OBJECTIVE: To study the dose-response relationship between lead and lead food products in whole blood and brain tissues of lead-poisoned mice and the content of antioxidant enzymes. Methods Seventy-five Kunming male mice weighing 18-22 g were randomly divided into the negative control group, the lead acetate model group and the lead-lead food low, medium and high dose groups. Except for the negative control group, the other groups were free to drink 1.00 gL-1 aqueous lead acetate solution for 3 and 0 d. The lead-discharged food groups were treated according to the human body recommended intake of 0.60g.kg-1.d-1 of 51,0 and 20 times by oral gavage; negative control group and lead acetate model group, gavage deionized water. After 1 month, the contents of trace elements (Ca, Pb, Fe, Cu, Zn) and superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) Aldehyde (MDA) content. Results The content of Pb, Ca, Cu and MDA in whole blood and brain tissue of lead-poisoned mice decreased with the increase of the dose of lead-lead food. The activities of Fe, Zn, SOD and GSH-PX increased with the increase of the dose of lead- increase. Conclusion The lead food can effectively regulate the balance of trace elements in whole blood and brain tissue, increase the activity of antioxidant enzymes and reduce the production of lipid peroxidation.