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应用体外培养、免疫细胞化学和计算机辅助图象分析方法研究了少突胶质细胞表达髓鞘碱性蛋白及高钾离子(30mmol/L)对少突胶质细胞表达髓鞘碱性蛋白的影响。结果表明,体外培养条件下,少突胶质细胞最早表达髓鞘碱性蛋白是在培养第7天。髓鞘碱性蛋白阳性的少突胶质细胞可分为大、中、小和双核四种类型,它们的构成比随培养时间延长呈动态变化。高K+不影响少突胶质细胞表达髓鞘碱性蛋白。实验结果提示,体外培养条件下,少突胶质细胞仍能正常表达髓鞘碱性蛋白,且表达时程并不依赖神经元的存在;细胞外K+浓度升高对髓鞘碱性蛋白在少突胶质细胞内的正常合成及表达过程无明显影响。本文对少突胶质细胞表达髓鞘碱性蛋白后的体积变化与其与中枢神经系统髓鞘形成的关系进行了讨论。
The effect of oligodendrocyte-derived myelin basic protein and high potassium (30mmol / L) on the expression of myelin basic protein in oligodendrocytes was studied by in vitro culture, immunocytochemistry and computer-aided image analysis . The results showed that under the conditions of in vitro culture, oligodendrocytes first expressed myelin basic protein on the 7th day of culture. Myelin basic protein-positive oligodendrocytes can be divided into four types, large, medium, small and dual-core, their composition ratio with the culture time showed a dynamic change. High K + does not affect oligodendrocyte expression of myelin basic protein. The experimental results suggest that oligodendrocytes can express myelin basic protein in vitro under the condition of in vitro culture, and the time course of expression is independent of the presence of neurons. The increase of extracellular K + concentration on myelin basic protein The normal synthesis and expression of the process in the oligodendrocytes had no significant effect. This article discusses the relationship between the changes of myelin basic protein expression in oligodendrocytes and myelination of central nervous system.