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目的:建立清热解毒口服液中4种主要活性成分的一测多评含量测定方法。方法:以黄芩苷为内标物,采用高效液相色谱法,建立其与其他3种成分绿原酸、连翘苷、栀子苷的相对校正因子,并用该校正因子进行计算,实现一测多评;同时采用外标法对4种成分进行含量测定,通过比较两法结果验证该方法的准确性。结果:建立的校正因子重复性较好,采用校正因子计算的含量值与外标法实测值偏差较小。结论:以黄芩苷、绿原酸、连翘苷、栀子苷为指标,采用一测多评法控制清热解毒口服液制剂的质量是可行的。
Objective: To establish a method for the determination of four major active ingredients in Qingrejiedu Oral Liquid. Methods: Baicalin was used as internal standard, and the relative calibration factors of chlorogenic acid, forsythin and geniposide were established with high performance liquid chromatography (HPLC) and calculated with the calibration factor Multiple evaluation; At the same time using the external standard method for the determination of four components, by comparing the results of two methods to verify the accuracy of the method. Results: The correction factor established was good repeatability, and the content calculated by the correction factor had little deviation from the actual value of external standard method. Conclusion: Using baicalin, chlorogenic acid, forsythin and geniposide as indexes, it is feasible to use one test to evaluate the quality of Qingrejiedu Oral Liquid.