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用电镜和细胞化学技术对毛竹[Phyllostachys edulis(Carr.)H.De Lehaie]节部韧皮部结“发育过程中Ca2+-ATP酶进行了超微细胞化学定位研究.结果显示:在韧皮部结”形成期,仅细胞质膜和细胞核上具有很高的Ca2+-ATP酶活性;随着韧皮部结“的发育,发育期细胞质膜上的Ca2+-ATP酶活性较形成期有所降低,而细胞核上仍保持较高的Ca2+-ATP酶活性,胞间连丝、运输小泡膜上都具有Ca2+-ATP酶活性;发育后期,液泡膜及内质网上也开始出现Ca2+-ATP酶沉积物;成熟期的韧皮部结”细胞质膜上的Ca2+-ATP酶活性较发育期有所升高,并且在韧皮部结“成熟的过程中,细胞核、内质网、胞间连丝、质体膜和细胞质降解物上始终都有较高的Ca2+-ATP酶活性.实验结果表明韧皮部结”细胞具有活跃的生理代谢以及频繁的共质体运输和信息交流.
The ultrastructure of Ca2 + -ATPase in Phyllostachys edulis (Carr.) H. De Lehaie nodules during the development of phloemostachys edulis (Carr.) H.De Lehaie was studied by electron microscopy and cytochemistry. The results showed that: Ca2 + -ATPase activity in the plasma membrane of the developmental stage was lower than that in the formation stage, while the development of the phloem junction “ Ca2 + -ATPase activity was maintained in Ca2 + -ATPase, Ca2 + -ATPase, Ca2 + -ATPase in the transport vesicle membrane. Ca2 + -ATPase deposition also began to appear on the vacuole membrane and endoplasmic reticulum in the later developmental stages. At the mature stage Phloem nodules ”Ca2 + -ATPase activity on the plasma membrane increased compared with the developmental period, and in the phloem junction “ maturation process, the nucleus, endoplasmic reticulum, plasma membrane, the plasma membrane and cytoplasmic degradation products Has always had a high activity of Ca2 + -ATPase.Experimental results show that phloem junction ”cells have active physiological metabolism and frequent exchange of sympathetic transport and information.