分别以间苯二酚和4-氯间苯二酚为原料,合成了两种香豆素类亚硫酸氢根离子探针——4-甲基-8-甲酰基-7-羟基香豆素和4-甲基-8-甲酰基-7-羟基-6-氯香豆素,并用IR、1HNMR、MS等方法对合成产物进行了表征。研究了该亚硫酸氢根离子探针的检测机理、选择性、方法的分析特性及其在实际样品检测中的应用,建立了一种在水溶液中检测亚硫酸氢根离子的荧光分析方法。 Resorcinol and 4-chlororesorcinol were used as raw materials to synthesize two coumarin-bisulfite ion probes, 4-methyl-8-formyl-7-hydroxycoumarin And 4-methyl-8-formyl-7-hydroxy-6-chlorocoumarin, and the synthesized products were characterized by IR, 1HNMR, MS and other methods. The detection mechanism, selectivity, analytical characteristics of the method and its application in the actual sample detection were studied. A fluorescence analysis method for detecting bisulfite ions in aqueous solution was established.