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采用两种识别不同抗原决定簇的抗尿激酶(UK)单克隆抗体建立了测定人 UK 含量的夹心法ELISA.本法灵敏度为0.15ng/ml,批内 CV4.3%,批间 CV8.7%,平均回收率98%.应用本法测定了部分正常细胞和肿瘤细胞株培养上清中的 UK 含量,肿瘤细胞培养上清 UK 量明显高于正常细胞.测定82名正常人血浆中 UK 含量,其结果为1.31±0.6ng/ml.
Sandwich ELISA for the determination of human UK content was established using two anti-urokinase (UK) monoclonal antibodies that recognize different antigenic determinants.The sensitivity of this method was 0.15 ng / ml with an intra-assay CV of 4.3% and inter-assay CV8.7 %, The average recovery rate of 98% .Application of this method was measured in some of the normal cells and tumor cell lines in the culture supernatant of the UK content of UK tumor cell culture supernatant was significantly higher than normal cells. Determination of 82 normal plasma UK content , The result was 1.31 ± 0.6 ng / ml.