论文部分内容阅读
目的观察红细胞生成素激活的星形胶质细胞条件培养液(EACM)对神经干细胞促分化作用及对分化后细胞的保护作用。方法原代培养神经干细胞和1型星形胶质细胞,收集红细胞生成素(EPO)刺激的星形胶质细胞上清液,用于神经干细胞分化实验的研究。对分化后的细胞进行免疫细胞化学染色,计算其分化为神经元的比率;同时利用FeSO4和H2O2制造细胞损伤模型,用EACM继续培养48h,然后检测细胞活性和存活细胞数。结果EACM组神经干细胞分化明显,神经元比率较星形胶质细胞上清组和对照组均有明显增加。分化后的细胞中加入H2O2后,继续用EACM培养的实验组吸光度(A)值和细胞存活百分比均较对照组高。结论红细胞生成素激活的星形胶质细胞条件培养液对神经干细胞有促其向神经元分化的作用,并对分化后的细胞有保护作用。
Objective To observe the effect of erythropoietin-activated astrocyte conditioned medium (EACM) on the differentiation and differentiation of neural stem cells and the protective effect on the differentiated cells. Methods Primary cultured neural stem cells and type 1 astrocytes were collected and stimulated with erythropoietin (EPO) for astrocyte supernatants for neural stem cell differentiation. The differentiated cells were immunocytochemically stained and the percentage of neurons differentiated into neurons was calculated. At the same time, cell injury models were made by FeSO4 and H2O2, and cultured with EACM for 48 hours. The cell viability and cell viability were measured. Results The differentiation of neural stem cells in EACM group was obvious, and the ratio of neurons was significantly higher than that in astrocyte supernatant group and control group. After adding H2O2 to the differentiated cells, the absorbance (A) and cell survival percentage of the experimental group cultured with EACM were higher than those of the control group. CONCLUSION: Erythropoietin-activated astrocyte conditioned medium can promote the differentiation of neural stem cells into neurons and protect the differentiated cells.