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利用RAPD和微卫星标记技术,对芙蓉鲫及其原始亲本(红鲫、芙蓉鲤、散鳞镜鲤、兴国红鲤)5个群体的遗传关系进行研究。利用20条随机引物进行RAPD分析,其中12条在5个群体中均能扩增出特异条带。统计分析显示:芙蓉鲫与红鲫、芙蓉鲤、散鳞镜鲤、兴国红鲤的遗传相似性分别为0.8757、0.7478、0.7419、0.7449,遗传距离分别为0.1327、0.2906、0.2985、0.2944。在进行微卫星标记分析时,所扩增的29对微卫星引物中共有13对在5个群体中均能扩增出特异条带。统计分析显示,芙蓉鲫与红鲫、芙蓉鲤、散鳞镜鲤、兴国红鲤的遗传相似性分别为0.8717、0.7434、0.6680、0.7552,遗传距离分别为0.1374、0.2965、0.4034、0.2808。两种分析结果均表明,芙蓉鲫的遗传结构更接近于父本红鲫,与外祖父兴国红鲤的遗传相似性比与外祖母散鳞镜鲤的遗传相似性要大,其遗传结构更偏向于具红色体征的原始亲本。
Using RAPD and microsatellite markers, the genetic relationships among five populations of Furong crucian carp and its original parents (red crucian carp, hibiscus carpio, scattered scales mirror carp and Xingguo red carp) were studied. Twenty random primers were used for RAPD analysis, of which 12 bands could be amplified in 5 populations. Statistical analysis showed that the genetic similarities between Hibiscus carpio, Carassius auratus, Furong carp, Scattered mirror carp and Xingguo red carp were 0.8757,0.7478,0.7419,0.7449 respectively, and the genetic distances were 0.1327,0.2906,0.2985,0.2944 respectively. In the microsatellite marker analysis, a total of 13 pairs of amplified 29 pairs of microsatellite primers could amplify specific bands in 5 populations. Statistical analysis showed that the genetic similarities between Furong carp and red crucian carp, Furong carp, Scattered mirror carp and Xingguo red carp were 0.8717,0.7434,0.6680,0.7552 and genetic distances were 0.1374,0.2965,0.4034,0.2808 respectively. The results of both analyzes showed that the genetic structure of Hibiscus carpio was closer to that of the male parent Carassius auratus gibelio, and the genetic similarity to the grandfather Xingguo red carp was greater than that of the grandfather Xingguo carp and the genetic structure was more biased The original sign of red signs.