目的:探讨中药化纤方对人胚肺二倍体纤维母细胞(2BS)增殖的抑制作用及其机理,为中医药介入肺纤维化防治提供实验和理论支持。方法:采用中药血清药理学实验方法,观察化纤方含药血清对2BS细胞的细胞增殖、细胞凋亡和细胞周期的影响。结果:经化纤方含药血清作用后,2BS细胞增殖受到显著抑制,并出现明显的细胞凋亡现象;化纤方各组S期细胞数显著增加,而G2/M期细胞数则明显下降,提示该方药可阻抑S期细胞向G2/M期的移行,从而抑制其分裂增殖。结论:化纤方有明显的抗肺纤维化作用,其作用机制可能是阻抑细胞增殖周期并诱导其发生细胞凋亡。 OBJECTIVE: To investigate the inhibitory effect of traditional Chinese chemical fiber prescription on the proliferation of human embryonic lung diploid fibroblasts (2BS) and its mechanism, providing experimental and theoretical support for the prevention and treatment of pulmonary fibrosis with traditional Chinese medicine. Methods: The serum pharmacology experiment method of traditional Chinese medicine was used to observe the effects of serum containing Huaxian Recipe on cell proliferation, cell apoptosis and cell cycle of 2BS cells. RESULTS: The proliferation of 2BS cells was significantly inhibited and the cell apoptosis was evident after the treatment with Huaxianxianfang serum. The number of cells in the S phase of each group of chemical fiber increased significantly, while the number of cells in the G2/M phase decreased significantly. The drug can inhibit the migration of S phase cells to G2/M phase, thereby inhibiting its proliferation. Conclusion: Huaxianfang has obvious anti-fibrotic effect, and its mechanism may be to inhibit the cell proliferation cycle and induce its apoptosis.