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目的 建立大鼠Alzheimer病 (AD)炎症病理模型 ,探讨吲哚美辛在AD中的防治作用。 方法 在大鼠双侧海马CA1区定向注射 β 淀粉样蛋白 (Aβ)作为模型组 ,注射生理盐水作为对照组 ,吲哚美辛灌胃治疗模型大鼠 ,以蒸馏水作治疗对照。 2周后各组大鼠均作Y型迷宫测试 ,然后对大鼠海马进行HE、刚果红、尼氏染色及免疫组化检测等病理学观察。采用方差分析对量化指标行统计分析。 结果 吲哚美辛显著抑制Aβ诱导的大鼠海马胶质细胞增生 ,具有白细胞介素 1β(IL 1β)高表达、神经元丧失以及空间学习记忆能力减退等效应 ;检测各组大鼠海马CA1区锥体细胞带损伤长度显示模型组和生理盐水对照组分别为 (1 80 8± 0 2 89)和 (0 378± 0 0 6 2 )mm ,吲哚美辛治疗组和治疗对照组分别为 (0 76 2± 0 15 3)和 (1 82 2± 0 2 78)mm ,(P <0 0 1)。 结论 在体条件下吲哚美辛显著抑制Aβ诱导的海马炎症损伤效应 ,支持AD的抗炎治疗假说
Objective To establish the pathological model of Alzheimer’s disease (AD) in rats and explore the preventive and therapeutic effects of indomethacin in AD. Methods Aβ was injected into CA1 area of hippocampus of rats as model group. Normal saline was injected into control group. Indomethacin was given to rats by intragastric administration, and distilled water was used as control. After 2 weeks, all the rats in each group were tested for Y-maze. Then the hippocampus of rats were observed with HE, Congo red, Nissl staining and immunohistochemistry. ANOVA analysis of quantitative indicators of the line. Results Indomethacin significantly inhibited Aβ-induced hippocampal astrocyte proliferation, with high expression of IL-1β, loss of neurons and loss of spatial learning and memory ability. Detection of hippocampal CA1 region The lengths of pyramidal cells with injury showed that the model group and the saline control group were (1808 ± 0 2 89) and (0 378 ± 0 0 6 2) mm, respectively. The indomethacin group and the control group were ( 0 76 2 ± 0 15 3) and (1 82 2 ± 0 2 78) mm (P 0 01). Conclusions Indometacin significantly inhibits the Aβ-induced injury of hippocampal inflammatory cytokines in vivo and supports the anti-inflammatory hypothesis of AD