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目的观察大鼠实验性脑挫伤后CNS内β-APP蛋白表达时空性规律。方法采用自由落体撞击法致大鼠脑挫伤模型,用免疫组化技术观察伤后β-APP蛋白在不同时间(0.5h、2h、6h、12h、24h、3d、7d、14d)的表达情况,并用计算机图像分析技术作定量统计分析。结果(1)皮质内β-APP蛋白在伤后表达呈一定波动性,0.5h表达明显增加后,逐渐下降,12h降至最低,低于对照组,随后再次表达增加,3d达高峰;(2)伤后24h海马区β-APP表达开始逐渐下降,3d降至最低,14d回到对照组水平;(3)齿状回(DG)内β-APP蛋白在伤后12h低于对照组。结论实验性脑挫伤后皮质β-APP表达时序性规律可为脑损伤时间推断提供一个新的参考指标,但海马区及DGβ-APP变化规律在脑损伤时间推断方面价值不大。
Objective To observe the temporal and spatial regularity of β-APP expression in CNS after experimental brain contusion in rats. Methods The rat model of cerebral contusion induced by free-fall shock was used to observe the expression of β-APP protein at different times (0.5h, 2h, 6h, 12h, 24h, 3d, 7d, 14d) And using computer image analysis technology for quantitative statistical analysis. Results (1) The expression of β-APP protein in the cortex showed a certain degree of fluctuation after injury. The expression of β-APP protein in the cortex gradually decreased after 0.5h, decreased gradually to the lowest level at 12h, then decreased again and reached the peak at 3d ) Β-APP began to decline gradually in hippocampus 24 hours after injury, 3d was reduced to the lowest level, and returned to the control level on the 14th day. (3) β-APP protein in dentate gyrus (DG) was lower than that in the control group 12h after injury. Conclusions The temporal regularity of β-APP expression in cortex after experimental contusion may provide a new reference index for inferring the time of brain injury. However, the changes of DGβ-APP in hippocampus and DGβ-APP are of little value in estimating the time of brain injury.