DETERMINING THE CONTENT OF JASMINODIN IN MEI RONG CAPSULE WITH THIN LAYER CHROMATOGRAPHY

来源 :Journal of Xi'an Medical University | 被引量 : 0次 | 上传用户:guanshui6
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Objective Jasminodin in Mei Rong capsule was determined by the method or thin layer chromatog- raphy. Mothods The sample Jasminodin was extracted with methanol and then the extract was chromatographed on silica gel GF254 plate with chloroform-methanol-water(10:3:1) as mobile phase and the chromatograms were scanned by using Shimadzu CS-930 dual wavelength TLCS in zegzag scanning mode at λ_s 370nm and λR 250nm. Results Callbration graph was rectilinear between 2.7μg~16.2μg per spot for the jasminodin. Conclusion Satisfa- tory results were obtained with the average recovery ratio of 99.76%,RSD 2.27%. Objective Jasminodin in Mei Rong capsule was determined by the method or thin layer chromatog-raphy. Mothods The sample Jasminodin was extracted with methanol and then the extract was chromatographed on silica gel GF254 plate with chloroform-methanol-water (10: 3: 1) as mobile phase and the chromatograms were scanned by using Shimadzu CS-930 dual wavelength TLCS in zegzag scanning mode at λ_s 370 nm and λR 250 nm. Results Callback graph was rectilinear between 2.7 μg ~ 16.2 μg per spot for the jasminodin. were obtained with the average recovery ratio of 99.76%, RSD 2.27%.
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