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目的 探讨少突胶质细胞对PC1 2细胞JAK2基因表达的影响及其意义。方法 用分离培养的少突胶质细胞及其细胞碎片分别作用于培养的PC1 2细胞 ,于不同作用时相点在观察PC1 2细胞突起变化的同时检测PC1 2细胞内JAK2mRNA的表达及JAK2蛋白的磷酸化。结果 在给予少突胶质细胞及其细胞碎片处理后 1 0min ,JAK2mRNA表达较对照组有显著增高 (P <0 .0 5) ,但处理 1 2h后 ,其表达虽较处理 1 0min及 4h有显著下降 ,但仍显著高于对照组 (P <0 .0 5)。对照组JAK2蛋白的活性较低 ,但少突胶质细胞及其细胞碎片加入后 ,JAK2蛋白很快便被磷酸化而激活 ,到处理后 1 2h ,激活的蛋白虽较处理 1 0min有显著下降 ,但仍显著高于正常对照组 (P <0 .0 5)。少突胶质细胞及其细胞碎片对其影响差别不显著 (P >0 .0 5)。结论 少突胶质细胞作用于PC1 2细胞时可调节PC1 2细胞JAK2的表达与激活 ,这种表达与激活在一定程度上可能介导少突胶质细胞对PC1 2细胞突起生长的影响。
Objective To investigate the effect of oligodendrocyte on the expression of JAK2 gene in PC12 cells and its significance. Methods The cultured oligodendrocytes and their cell debris were respectively used to act on cultured PC12 cells. The expression of JAK2 mRNA in PC12 cells and the expression of JAK2 protein Phosphorylation. Results The expression of JAK2 mRNA was significantly increased at 10 min after oligodendrocyte and cell debris treatment (P <0.05), but the expression of JAK2 mRNA was significantly higher at 10 min and 4 h after treatment Significantly decreased, but still significantly higher than the control group (P <0.05). The activity of JAK2 protein in the control group was lower. However, after the oligodendrocytes and its cellular debris were added, the JAK2 protein was rapidly phosphorylated and activated. At 12 h after the treatment, although the activated protein had a significant decrease compared with 10 min , But still significantly higher than the normal control group (P <0.05). The effect of oligodendrocyte and its cellular debris was not significant (P> 0.05). Conclusion When oligodendrocytes act on PC12 cells, the expression and activation of JAK2 in PC12 cells can be regulated. The expression and activation of oligodendrocytes may mediate the influence of oligodendrocytes on the growth of PC12 cells to a certain extent.