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利用菌丝生长速率法,测定了自辽宁省主产稻区2009-2010年分离的87株稻瘟病菌对稻瘟灵的敏感性。结果表明,稻瘟灵的抑制中浓度EC50值为0.35～7.01μg/mL,敏感性差异达20倍;且2009年稻瘟病菌对稻瘟灵的敏感性高于2010年。根据野生敏感型病原群体对药剂敏感性呈正态分布的原理,辽宁省稻瘟病菌对稻瘟灵的敏感性基线定为(1.77±0.80)μg/mL,辽宁省主要稻区均有抗稻瘟灵菌株出现,除2010年在铁岭稻产区监测到1株中抗菌株外,大部分稻瘟病菌株都表现为低抗水平。稻瘟病菌不同生理小种群对稻瘟灵的敏感性无明显差别。
The mycelia growth rate method was used to determine the sensitivity of 87 isolates of Magnaporthe grisea to rice blast isolates from 2009 to 2010 in the main producing rice of Liaoning Province. The results showed that the inhibitory concentration of rice blast was EC50 0.35 ~ 7.01μg / mL, the sensitivity of up to 20 times; and in 2009 Magnaporthe griseus blastao sensitivity higher than in 2010. According to the principle that the sensitivity of wild-sensitive pathogens to the drug is normally distributed, the baseline sensitivity of M. grisea to rice blast in Liaoning Province was (1.77 ± 0.80) μg / mL, and the main rice-growing areas in Liaoning Province had anti-rice Blasting strains appeared, except for one of the medium-resistant strains tested in Tieling rice-producing area in 2010, most of the rice blast strains showed low anti-level. There was no significant difference in susceptibility to rice blast between different physiological populations of Magnaporthe grisea.