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用有 HBV 复制、抗-HBc 阳性的乙肝病人的血清,经除垢剂处理后可测得 HBcAg。这一现象解释为含有 Dane 颗粒的免疫复合物的解离而并非 Dane 颗粒的变性。在作者的实验室曾以重组 HBcAg 和抗-HBc 体外研究证明免疫复合物形成并可被硫氰化钠解离,解离后的-HBcAg 被放射免疫法检出而不伴有抗原性的消失。病人和方法 10例 HBV 感染后不出现或延迟出现抗-HBc 的病人,其中4例急性乙肝,6例为 HBeAg阳性的 HBsAg 携带者并伴慢性持续性肝炎至少2年,所有病人均被随访数月至5年。10例中4例急性乙肝
With HBV replication, anti-HBc-positive serum of patients with hepatitis B, after descaling treatment can be measured HBcAg. This phenomenon is explained by the dissociation of immune complexes containing Dane particles rather than the denaturation of Dane particles. In the authors’ lab, in vitro studies with recombinant HBcAg and anti-HBc have demonstrated that immune complexes are formed and can be dissociated by sodium thiocyanate, and the dissociated -HBcAg is detected by radioimmunoassay without loss of antigenicity . PATIENTS AND METHODS Ten patients with HBV who did not develop or delay anti-HBc after HBV infection were enrolled. Among them, 4 were acute hepatitis B and 6 were HBeAg-positive HBsAg carriers with chronic persistent hepatitis for at least 2 years. All patients were followed up Month to 5 years. 4 cases of acute hepatitis B in 10 cases