论文部分内容阅读
目的:探讨环氧合酶2(COX-2)抑制剂塞来昔布对人腺样囊性癌细胞株(SACC-M)增殖和侵袭的抑制作用。方法:用CCK-8法检测不同浓度塞来昔布作用后SACC-M的增殖能力;用Western Blotting技术,比较塞来昔布作用24 h后的SACC-M中COX-2的表达;采用Transwell小室方法,检测塞来昔布作用24 h后的SACC-M的侵袭能力。结果:CCK-8法显示,塞来昔布对SACC-M细胞增殖有明显抑制作用,并表现出剂量依赖性。侵袭抑制实验表明,塞来昔布对SACC-M细胞的侵袭能力有抑制作用,与对照组SACC-M细胞相比较,有显著差异,其抑制作用具有浓度依赖性。Western Blotting实验得出,经过塞来昔布作用24 h后,SACC-M细胞中COX-2蛋白表达水平随塞来昔布浓度的升高依次降低。结论:塞来昔布对人腺样囊性癌细胞增殖与侵袭能力有明显抑制作用。
Objective: To investigate the inhibitory effect of cyclooxygenase 2 (COX-2) inhibitor celecoxib on the proliferation and invasion of human adenoid cystic carcinoma cell line (SACC-M). Methods: The proliferation of SACC-M cells was detected by CCK-8 assay. The expression of COX-2 in SACC-M cells was detected by Western Blotting. Chamber method to detect the invasive ability of SACC-M after 24 h of celecoxib. Results: CCK-8 method showed that celecoxib inhibited the proliferation of SACC-M cells in a dose-dependent manner. Invasion inhibition experiments showed that celecoxib inhibited the invasion ability of SACC-M cells, compared with the control group SACC-M cells, there was a significant difference in its concentration-dependent inhibition. Western Blotting experiments showed that after 24 h of celecoxib, the expression of COX-2 protein in SACC-M cells decreased with the increase of celecoxib concentration. Conclusion: Celecoxib has a significant inhibitory effect on the proliferation and invasion of human adenoid cystic carcinoma cells.