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为探讨外源茉莉酸对青杨Populus cathayana的诱导抗性及其对舞毒蛾Lymantria dispar的影响,室内对青杨扦插苗喷施不同浓度的茉莉酸(对照为0.17%丙酮),分别在喷施后1,5和10d采集叶片分析其保护性酶活性的变化,并接舞毒蛾幼虫于青杨苗木上观测其长发育情况。结果表明:0.1和0.001mmol/L两种浓度的茉莉酸(JA)处理均使青杨叶过氧化物酶(POD)、多酚氧化酶(PPO)、苯丙氨酸解氨酶(PAL)、胰凝乳蛋白酶抑制剂(CI)和胰蛋白酶抑制剂(TI)活性较对照增加(P<0.05)。取食茉莉酸诱导的青杨苗木后,舞毒蛾幼虫的发育历期延长,体重降低。0.1mmol/L茉莉酸诱导的青杨苗木5d后接虫,使舞毒蛾幼虫的发育历期显著延长,较对照长8d;接虫21d后称重时,取食茉莉酸诱导的青杨叶片的幼虫体重较对照组降低了50%～100%,该结果说明外源茉莉酸诱导青杨产生了对舞毒蛾明显的抗虫性。
In order to investigate the induction resistance of exogenous jasmonic acid to Populus cathayana and its effect on Lymantria dispar, different concentrations of jasmonic acid (0.17% acetone) were sprayed on the cuttings of Populus euphratica, The changes of protective enzyme activity were analyzed after 1, 5, and 10 days, and the larvae of Gypsy moth were observed for their long-term development on Populus seedlings. The results showed that the activities of POD, PPO, PAL were significantly increased after treated with 0.1 and 0.001 mmol / L JA, , The activity of chymotrypsin inhibitor (CI) and trypsin inhibitor (TI) increased compared with the control (P <0.05). After feeding jasmonic acid-induced Poplar seedlings, gypsy moth larvae developmental duration, weight loss. 0.1 mg / L jasmonic acid-induced poplar seedlings 5 days after the pick worm, the Gypsy moth larvae developmental duration significantly longer than the control 8d; pick 21d after weighing, feeding jasmonic acid-induced poplar leaves The body weight of larvae was reduced by 50% ~ 100% compared with that of the control group. The results showed that the exogenous jasmonic acid induced Populus euphratica had obvious insect resistance to Gypsy moth.