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本研究以刚毛柽柳种子为材料,对0 mmol/L(对照)、50 mmol/L、100 mmol/L、150 mmol/L、200 mmol/L NaCl胁迫下柽柳种子的发芽率、发芽势和发芽指数进行计算,结果表明:对照组的发芽率、发芽势和发芽指数分别为97.00%、41.56%与28.25%,100 mmol/L NaCl胁迫下的发芽率、发芽势及发芽指数分别为54.00%、24.25%和18.50%,接近对照组的50%,因此设定100 mmol/L NaCl浓度为柽柳种子萌发的盐阈值。在100 mmol/L NaCl胁迫下,研究不同浓度梯度的细胞分裂素(6-benzylaminopurine,6-BA)、赤霉素(gibberellic acid,GA3)、褪黑素(melatonine,MT)、萘乙酸(1-naphthylacetic acid,NAA)对柽柳种子发芽率、发芽势和发芽指数的影响。结果表明,NaCl胁迫下,浓度为10 mg/L的6-BA和100 ng/L的GA3对柽柳种子萌发的抑制的缓解效应较明显,发芽率分别达到81.50%和94.50%;浓度为32μg/L的NAA与400 mmol/L的MT对柽柳种子萌发的抑制的加剧效应较明显,发芽率分别为42.50%和41.50%。本研究为提高NaCl胁迫下柽柳种子的萌发繁殖提供实际指导,也为盐胁迫下利用激素对柽柳种子萌发的控制与缓解提供了借鉴与参考。
In this research, the seed germination, germination energy and germination of Tamarix chinensis seedlings under 0 mmol / L (control), 50 mmol / L, 100 mmol / L, 150 mmol / L and 200 mmol / The results showed that the germination rate, germination energy and germination index of the control group were 97.00%, 41.56% and 28.25%, respectively. The germination rate, germination energy and germination index under 100 mmol / L NaCl stress were 54.00% 24.25% and 18.50% respectively, which is close to 50% of the control group. Therefore, 100 mmol / L NaCl concentration was set as the salt threshold for tamarix seed germination. The effects of different concentrations of 6-benzylaminopurine (6-BA), gibberellic acid (GA3), melatonine (MT) -naphthylacetic acid (NAA) on the seed germination rate, germination potential and germination index of Tamarix. The results showed that under NaCl stress, 6-BA and 100 ng / L of GA3 with concentration of 10 mg / L significantly inhibited the seed germination of Tamarix sibirica, with the germination rates reaching 81.50% and 94.50% respectively; the concentration of 32μg / The aggravating effect of NAA with L and 400 mmol / L MT on the seed germination of Tamarix ramosissima was obvious, with germination rates of 42.50% and 41.50% respectively. This study provided practical guidance for improving the seed germination and propagation of Tamarix chinensis under salt stress and provided references for the control and alleviation of the seed germination of Tamarix chinensis under salt stress.