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目的 研究 HP感染与胃癌及癌前病变中环氧合酶 - 2 (cox- 2 )、p2 1表达的关系 ,以探讨 HP致癌的可能机制。方法 经胃镜和病理诊断明确的病例共 12 0例 ,包括慢性浅表性胃炎 (CSG)、肠上皮化生、不典型增生、胃癌各 30例。以 ABC免疫组化法检测上述标本的 cox- 2、p2 1,以改良 Giem sa法检测 HP。结果 胃癌组肠型胃癌 HP阳性率为 83.3% (2 0 / 2 4) ,弥漫型 6例中 HP阳性 1例。胃癌 HP阳性率与 CSG比较差异高度显著性 (P<0 .0 1) ,肠型胃癌 HP阳性率与胃癌阳性率比较有显著性差异 (P<0 .0 5 )。各组 HP阳性的 cox- 2及 p2 1与其 HP阴性者作相应比较均有显著或高度显著性差异 (P<0 .0 5～ 0 .0 1)。结论 提示胃癌可能与 HP感染有关 ,而 HP感染与肠型胃癌的关系更为密切 ,HP感染可使胃黏膜中 cox- 2过度表达 ,导致细胞凋亡受抑制 ,并通过使癌基因激活从而可能引起胃癌的发生
Objective To study the relationship between HP infection and the expression of cyclooxygenase-2 (COX-2) and p21 in gastric cancer and precancerous lesions to explore the possible mechanism of HP carcinogenesis. Methods A total of 120 cases were identified by gastroscopy and pathological diagnosis, including 30 cases of chronic superficial gastritis (CSG), intestinal metaplasia, atypical hyperplasia, and gastric cancer. The cox-2 and p21 of these samples were detected by ABC immunohistochemistry to detect HP using the modified Giem sa method. Results The positive rate of HP-positive gastric cancer in gastric cancer group was 83.3% (2 0 / 24), and the diffuse-type 6 cases were positive in HP. The positive rate of HP in gastric cancer was significantly higher than that in CSG (P<0.01). The positive rate of HP in intestinal type gastric cancer was significantly different from that in gastric cancer (P<0.05). Each group of HP-positive cox-2 and p21 was significantly or highly significantly different from its HP-negative group (P < 0.05 to 0.01). Conclusions suggest that gastric cancer may be related to HP infection, and HP infection is more closely related to intestinal type gastric cancer. HP infection may cause overexpression of cox-2 in gastric mucosa, leading to inhibition of apoptosis, and possibly by activating oncogenes. Cause gastric cancer