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目的：观察中脑导水管周围灰质（ＰＡＧ）的阿片受体和促皮质素（ＡＣＴＨ）在鞘内吗啡影响免疫功能时的作用和变化．方法：用铕标的靶细胞检测大鼠脾脏自然杀伤（ＮＫ）细胞活性，ＭＴＴ和结晶紫蓝法分析ＣｏｎＡ诱生的脾细胞白细胞介素２（ＩＬ２），肿瘤坏死因子ＴＮＦβ活性和血清ＴＮＦα水平，放免法测定血清促皮质素（ＡＣＴＨ）水平．结果：鞘内注射吗啡抑制脾ＮＫ细胞活性，ＣｏｎＡ诱生的ＩＬ２和ＴＮＦβ活性，并伴有血清ＡＣＴＨ水平的上升．ＰＡＧ微量注射纳洛酮部分拮抗吗啡引起的ＮＫ细胞活性的抑制，伴ＡＣＴＨ水平恢复．结论：ＰＡＧ内的阿片受体参与鞘内注射吗啡引起的ＮＫ细胞活性的抑制，同时伴有ＨＰＡ轴的激活
OBJECTIVE: To observe the effects and changes of opioid receptors and corticotropin (ACTH) in periaqueductal gray (PAG) on the immune function of intrathecal morphine. Methods: Natural killer (NK) cell activity of rat spleens was detected by europium-labeled target cells. Interleukin-2 (IL-2) and TNF-β activity of spleen cells induced by ConA were analyzed by MTT and crystal violet- And serum TNF α levels, radioimmunoassay serum corticotropin (ACTH) levels. Results: Intrathecal injection of morphine inhibited spleen NK cell activity, Con 2 induced IL 2 and TNF β activity, accompanied by an increase in serum ACTH levels. PAG microinjection of naloxone partially antagonized the inhibition of morphine-induced NK cell activity, with recovery of ACTH levels. CONCLUSION: The opioid receptors in PAG are involved in the inhibition of NK cell activity induced by intrathecal morphine injection, accompanied by HPA axis activation