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将温度敏感型p53(p53Val135)基因导入NIH3T3细胞进行过量表达, 发现在温度敏感型p53的活性温度(32℃)下培养, NIH3T3细胞迅速表现出与衰老相关的形态特征和生化特性. 这可能是p53过量表达激活了NIH3T3细胞内在的衰老机制从而诱导细胞进入衰老状态.
The temperature-sensitive p53 (p53Val135) gene was introduced into NIH3T3 cells for overexpression. It was found that NIH3T3 cells rapidly exhibit senescence-related morphological and biochemical characteristics when cultured at the temperature-sensitive p53 activity temperature (32°C). This may be Overexpression of p53 activates the intrinsic aging mechanism of NIH3T3 cells and induces the cells to enter the senescence state.