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目的：通过体内实验，探讨小柴胡汤（ＸＣＨＴ）浸膏对荷瘤鼠Ｓ１８０的抑制作用。方法：采用透射电镜观察小柴胡汤对瘤细胞生长和结构的影响。结果：高、中和低浓度ＸＣＨＴ（１０００ｇ·Ｌ－１、５００ｇ·Ｌ－１、２５０ｇ·Ｌ－１）溶液治疗后，对荷瘤鼠Ｓ１８０细胞均有明显抑制作用，抑瘤率４０１９％～５８７３％，其中以高浓度组疗效最佳。同时可见经ＸＣＨＴ治疗后各组Ｓ１８０细胞均出现凋亡的特征，表现为细胞体积变小，核固缩，胞浆和核染色质凝集，密度增高，细胞间有大量凋亡小体，其中以低浓度组为甚，而高浓度组Ｓ１８０细胞则以坏死为主。结论：ＸＣＨＴ对荷瘤鼠Ｓ１８０有明显抑制作用，可诱导Ｓ１８０细胞凋亡及坏死，其中瘤细胞以哪种死亡方式为主，依用药的剂量不同而异
Objective: To investigate the inhibitory effect of Xiao Chai Hu Tang (XCHT) extract on tumor-bearing mice S180 by in vivo experiments. Methods: Transmission electron microscopy was used to observe the effect of Xiaochaihu Decoction on the growth and structure of tumor cells. RESULTS: After treatment with high, medium, and low concentrations of XCHT (1000 g·L-1, 500 g·L-1, 250 g·L-1), the inhibition of S180 cells in tumor-bearing mice was evident. The inhibition rate was 4019. %～5873%, of which the highest concentration was the best. At the same time, after treatment with XCHT, all groups of S180 cells exhibited apoptotic characteristics, showing smaller cell size, nuclear condensation, agglutination of cytoplasm and nuclear chromatin, increased density, and a large number of apoptotic bodies between cells. The low concentration group was very severe, while the high concentration group S180 cells were mainly necrotic. Conclusion: XCHT has a significant inhibitory effect on S180 tumor-bearing mice, which can induce apoptosis and necrosis of S180 cells. The type of death depends mainly on the type of tumor cells, depending on the dose of the drug.