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目的探讨甲强龙对实验性变态反应性脑脊髓炎小鼠的协同刺激分子CD80和CD86的影响。方法实验性变态反应性脑脊髓炎(EAE)模型通过以髓鞘少突胶质细胞糖蛋白多肽35-55(MOG35-55)为抗原诱导。将30只雌性C57BL/6J小鼠随机分为甲强龙组、EAE组和正常组,每组10只。采用蛋白质印迹法检测大脑的CD80和CD86表达水平。结果甲强龙组神经功能缺损症状轻于EAE组,大脑CD80表达水平明显低于EAE组(P<0.05),而CD86表达明显高于EAE组(P<0.05)。结论甲强龙能改善EAE小鼠的症状,其作用机制之一是抑制CD80表达并上调CD86的表达。
Objective To investigate the effects of methylprednisolone on costimulatory molecules CD80 and CD86 in mice with experimental allergic encephalomyelitis. Methods Experimental allergic encephalomyelitis (EAE) model was induced by using myelin oligodendrocyte glycoprotein 35-55 (MOG35-55) as antigen. Thirty female C57BL / 6J mice were randomly divided into methylprednisolone group, EAE group and normal group, with 10 mice in each group. Western blotting was used to detect the expression of CD80 and CD86 in the brain. Results Compared with EAE group, the neurological deficits in Jiaqianglong group were lower than those in EAE group (P <0.05), while the expression of CD86 in brain was significantly higher than that in EAE group (P <0.05). Conclusions Methylprednisolone can ameliorate the symptoms of EAE mice. One of the mechanisms is that it inhibits the expression of CD80 and up-regulates the expression of CD86.