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目的 观察碱性成纤维细胞生长因子(bFGF)和硫糖铝联合治疗大鼠实验性胃溃疡的疗效。方法 取32只Sprage Dawley雌性大鼠,平均分为4 组。用bFGF 100 ng·100 g-1·d-1、硫糖铝40 mg·100g-1·d-1、bFGF 100 ng+硫糖铝40 mg·100g-1·d-1、生理盐水1 ml·100 g-1·d-1,分别等容灌胃治疗乙酸诱发的大鼠胃溃疡21 d,观测各组溃疡愈合情况及溃疡周围组织毛细血管增生情况,检测各组胃酸、胃蛋白酶含量。结果 bFGF组对胃酸、胃蛋白酶分泌的影响与生理盐水组相比无差异。bFGF组和硫糖铝组溃疡面积分别为(2.9±0.8) mm2、(3.2±1.1) mm2,明显小于生理盐水组(6.8±1.4) mm2(P<0.05), bFGF+硫糖铝组溃疡面积(2. 0 ±1.1) mm2,小于bFGF组和硫糖铝组(P<0.05)。bFGF组和bFGF+硫糖铝组溃疡边缘组织中毛细血管数分别为(19.7±3.0)、(21.9±4.5)条/每视野,明显多于硫糖铝组(12.2±2.2)条/每视野,生理盐水组(7.0±2.1)条/每视野(P<0.05)。结论 bFGF在不影响胃酸胃蛋白酶分泌情况下明显加速乙酸诱发大鼠胃溃疡的愈合,bFGF+硫糖铝效果更好。bFGF和bFGF+硫糖铝均能明显促进大鼠溃疡周围组织毛细血管增生。
Objective To observe the curative effect of combination of bFGF and sucralfate on experimental gastric ulcer in rats. Methods Thirty-two Sprage Dawley female rats were divided into four groups on average. BFGF 100 ng · 100 g-1 · d-1, sucralfate 40 mg · 100g-1 · d-1, bFGF 100 ng + sucralfate 40 mg · 100g-1 · d-1, saline 1 ml · 100 g-1 · d-1, respectively, the same volume of gastric ulcer treatment of acetic acid-induced gastric ulcer for 21 days to observe the healing of ulcers in each group and the capillary surrounding tissue of the ulcer, detect the content of gastric acid and pepsin in each group. Results bFGF group gastric acid, pepsin secretion no difference compared with the saline group. The ulcer area in bFGF group and sucralfate group was (2.9 ± 0.8) mm2, (3.2 ± 1.1) mm2, which was significantly lower than that in saline group (6.8 ± 1.4) mm2 (P <0.05) 2. 0 ± 1.1) mm2, less than bFGF group and sucralfate group (P <0.05). The number of capillary vessels in the bFGF group and the bFGF + sucralfate group was (19.7 ± 3.0) and (21.9 ± 4.5) bars / field, which was significantly higher than that in the sucralfate group (12.2 ± 2.2 bars / field) Physiological saline group (7.0 ± 2.1) / visual field (P <0.05). Conclusion bFGF significantly accelerates acetic acid-induced healing of gastric ulcer without affecting gastric acid pepsin secretion, and bFGF + sucralfate is more effective. bFGF and bFGF + sucralfate can significantly promote capillary proliferation around the ulcer in rats.