目的　探讨微丝在碱性成纤维细胞生长因子 (bFGF)调节伤口愈合中的作用。　方法　采用免疫荧光染色、显微荧光光度计及 3H -TdR掺入法研究bFGF诱导的成纤维细胞微丝骨架的变化 ,观察用细胞松弛素B破坏微丝后 ,bFGF对细胞运动及细胞增殖的影响。　结果　bFGF使成纤维细胞微丝骨架发生有序的变化 ;使F -肌动蛋白 (F -actin)含量增高 ,并呈一定的时间 -剂量依赖关系。用细胞松弛素B破坏微丝 ,可明显抑制由bFGF引起的细胞向创面的迁移 ,同时也显著抑制了bFGF引起的成纤维细胞 3H -TdR掺入量。　结论　bFGF可通过微丝骨架的变化影响创伤愈合过程中细胞的迁移、分化和增殖。 Objective To investigate the role of microfilament in the regulation of wound healing by basic fibroblast growth factor (bFGF). Methods The changes of fibroblast microfilament skeleton induced by bFGF were studied by immunofluorescence staining, microscopic fluorescence spectrophotometer and 3H-TdR incorporation. The effect of bFGF on the cell motility and cell proliferation influences. Results bFGF caused an orderly change in the microfilament cytoskeleton of Fibroblasts and increased the content of F-actin in a time-and-dose-dependent manner. Binding of cytochalasin B to actin filaments significantly inhibited the migration of cells to the wound caused by bFGF and significantly inhibited 3H-TdR incorporation by bFGF. Conclusion bFGF can influence the migration, differentiation and proliferation of wound during wound healing through the change of microfilament skeleton.