目的研究梭曼对急性中毒大鼠的自由基损伤作用,探讨维生素A、E的抗氧化作用。方法雄性大鼠,随机分为对照组、阳性组、VA组、VE组。灌胃10d,第11d除阴性组外其余各组大鼠均皮下注射0.9LD50梭曼,2h后取样,测定各组全血和肝组织的谷胱甘肽过氧化物酶GSH-Px、过氧化氢酶CAT、VE和总抗氧化力T-AOC。结果梭曼中毒2h后,GSH-Px活性、T-AOC水平、VE含量降低。维生素A、E均有效的抑制其变化。结论梭曼对大鼠有自由基损伤作用,维生素A、E对梭曼中毒大鼠过氧化损伤具有保护作用,提示维生素A、E对梭曼中毒有预防作用。 Objective To study the effect of soman on free radical damage in acute poisoning rats and explore the antioxidative effect of vitamin A and E. Methods Male rats were randomly divided into control group, positive group, VA group and VE group. The rats in the other groups were subcutaneously injected with 0.9 LD50 soman for 10 days and the rats in the other groups were sacrificed on the 11th day. After 2 hours, the GSH-Px, Hydrogenase CAT, VE and total antioxidant capacity T-AOC. Results Sohmann poisoning 2h, GSH-Px activity, T-AOC levels, VE decreased. Vitamin A, E are effective in inhibiting its changes. Conclusion Soman may have a damaging effect on free radicals in rats. Vitamin A and vitamin E have a protective effect on peroxide-induced oxidative damage in soman-induced rats, suggesting that vitamin A and vitamin E can prevent soman intoxication.