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目的采用自制的钉螺(Oncomelania hupensis)模拟装置法对比法,初步观察蛋白质对日本血吸虫毛蚴(Schistosoma japanicummiracidia)的趋向作用及其在“模拟钉螺”中的应用。方法采用自制的钉螺模拟装置——玻璃盲管,取钉螺模拟装置四支,标号为A、B、C、D。A管装满去氯水,B管装满螺SCW液,C管装满去离子的SCW液,D管装满去离子去蛋白质的SCW液。在玻璃平底圆盘中注入刚孵出的毛蚴悬液200 ml(毛蚴密度约20个/ml),静置3 min,让毛蚴在容器内自由活动并均匀分布。然后将钉螺模拟装置同时放入玻璃圆盘中,5 min后将装置同时取出,进行观察和计数比较。结果 B管与C管毛蚴数明显高于A管,且差异有统计学意义(P<0.05);C管与D管差异无统计学意义(P>0.05)。结论蛋白质分子对毛蚴的吸引作用很小,没有明显的排斥作用,也没有明显的吸引作用,在钉螺吸引毛蚴的理论方面可以不考虑蛋白质分子的趋向影响。
OBJECTIVE: To study the tendency of proteins on Schistosoma japanicummiracidia and its application in “Simulated snails” by using the homemade Oncomelania hupensis simulation device method. Methods A self-made snail simulator, glass blind tube and four snail simulators, labeled A, B, C and D, were used. Tube A is filled with dechlorinated water, tube B is filled with SCW liquid, tube C is filled with deionized SCW solution, tube D is filled with deionized deproteinized SCW solution. In a glass flat-bottomed disk, 200 ml of newly hatched miracial suspension (miracidium density of about 20 / ml) was injected and allowed to stand for 3 minutes to allow the miracidia to move freely and uniformly in the container. The snail simulator was then placed in the glass disk at the same time and the device was removed at the same time after 5 min for observation and counting comparison. Results The numbers of spider larvae in tube B and tube C were significantly higher than those in tube A, and the difference was statistically significant (P <0.05). There was no significant difference between tube C and tube D (P> 0.05). Conclusion The protein molecules have little attraction to miracidia, no obvious repulsion and no obvious attraction. The theory of snail attracting miracidosis can not consider the tendency of protein molecules.