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目的分别考察橙皮苷在大鼠各肠段的吸收动力学特征及不同药物浓度、不同pH值灌流液对其吸收的影响。方法采用大鼠在体肠单向灌流实验方法,用HPLC对灌流液中的橙皮苷进行分析。结果橙皮苷在肠道中的吸收按回肠、空肠、十二指肠、结肠的顺序依次下降,其中在结肠中的吸收显著低于其他肠段(P<0.05);pH5.0、6.8的酸性条件下肠道对橙皮苷的吸收显著高于pH7.8弱碱条件(P<0.05);随着橙皮苷浓度的增加其Ka和Papp均逐渐下降,其中质量浓度为2mg·L-1的溶液Papp显著高于其他2个浓度(P<0.05)。结论橙皮苷在全肠段均有吸收,但在回肠中吸收较好;弱酸性环境易于橙皮苷吸收;橙皮苷在大鼠肠道的吸收存在饱和现象,其在机体内的转运可能存在主动转运或促进扩散。
Objective To investigate the absorption kinetics of hesperidin in various intestine of rats and the effect of different concentrations of perfusate on its absorption. Methods The rat intestine intestinal perfusion experiment was used to analyze hesperidin in perfusate. Results The absorption of hesperidin in the intestine decreased in the order of ileum, jejunum, duodenum and colon, with the absorption in the colon being significantly lower than that in other bowel segments (P <0.05); the acidity (P <0.05). With the increase of hesperidin concentration, Ka and Papp decreased gradually, and the concentration of hesperidin was 2 mg · L-1 Papp was significantly higher than the other two concentrations (P <0.05). Conclusion Hesperidin is absorbed in the whole intestine but absorbed well in the ileum. Hesperidin is easily absorbed in the weakly acidic environment. Hesperidin is saturated in the intestine of rats and its transport in the body may be There is active transport or promote diffusion.