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建立一种同时测定杨树花和杨树叶中豆甾醇、麦角甾醇、β-谷甾醇含量的反相高效液相色谱法。方法:以甲醇为流动相,测定杨树花和杨树叶中豆甾醇、麦角甾醇、β-谷甾醇的含量。结果:杨树花和杨树叶中3个植物甾醇的线性范围为0.00008~0.08μmol·m L-1,r>0.9990。杨树花中麦角甾醇、豆甾醇、β-谷甾醇的加样回收率分别在92.6%~100.0%、93.9%~100.1%、94.0%~100.0%之间,RSD(n=6)分别为2.8%、2.2%、2.8%;杨树叶中各甾醇加样回收率分别在91.3%~100.0%、91.2%~100.0%、92.5%~100.1%之间,RSD(n=6)分别为2.7%、2.7%、2.5%。结论:该方法稳定、快速,精密度高,线性范围宽,适合杨树花和杨树叶中的豆甾醇、麦角甾醇、β-谷甾醇含量测定。
To establish a simultaneous determination of stigmasterol, ergosterol and β-sitosterol in poplar and poplar by reversed-phase high-performance liquid chromatography. Methods: The content of stigmasterol, ergosterol and β-sitosterol in poplar and poplar leaves was determined by using methanol as the mobile phase. Results: The linear range of three phytosterols in poplar and poplar leaves was 0.00008 ~ 0.08μmol · m L-1, r> 0.9990. The recovery rates of ergosterol, stigmasterol and β-sitosterol in poplar were 92.6% -100.0%, 93.9% -100.1% and 94.0% -100.0%, respectively. The recoveries of RSD (n = 6) were 2.8% 2.2% and 2.8%, respectively. The recoveries of various sterols in poplar leaves ranged from 91.3% to 100.0%, from 91.2% to 100.0% and from 92.5% to 100.1%, respectively. RSDs were 2.7% and 2.7% , 2.5%. Conclusion: The method is stable, rapid, accurate and linear. It is suitable for the determination of stigmasterol, ergosterol and β-sitosterol in poplar and poplar leaves.