目的:研究祛白片对白癜风小鼠模型的疗效及其相关作用机制。方法:采用5%H2O2建立小鼠白癜风模型,实验设空白对照组、模型对照组和高、中、低剂量给药组。肉眼观察各组小鼠皮肤色泽变化及其毛发生长情况,同时采用HE染色法,镜下观察皮肤组织形态学改变。采用全基因芯片测试方法探讨祛白片的作用机制。结果:整体动物实验结果显示实验高、中、低剂量组与模型组相比疗效有统计学差异,各剂量组间具有浓度依赖性。HE染色结果显示,与模型组相比,高、中剂量组小鼠皮肤受试区出现大量黑色素颗粒,同时毛囊数恢复。小鼠全基因芯片研究结果显示,给药组与模型组相比共2 219个基因转录有变化,这些差异基因分别对文献报导的7种白癜风发病机制中的4种机制有调控作用。初步确认祛白片的作用机制与抑制自身免疫系统亢进、促进角质细胞形成、降低氧化应激反应和促进黑色素形成相关。结论:本研究证实了祛白片的抗白癜风作用,同时对其分子机制进行了初步探索,为后期深一步研究提供方向。 Objective: To study the curative effect of Qubai Tablets on vitiligo mouse model and its related mechanism. Methods: The mouse model of vitiligo was established by using 5% H2O2. The blank control group, model control group and high, medium and low dose groups were established. Macroscopically observed the skin color changes and the hair growth of mice in each group, meanwhile, the morphological changes of the skin tissue were observed by HE staining. The whole gene chip test method to explore the mechanism of Qu Bai tablets. Results: The overall animal experiments showed that the experimental high, medium and low dose groups compared with the model group, the efficacy was statistically significant, and dose-dependently. HE staining showed that compared with the model group, a large number of melanin granules appeared in the test area of ​​high and medium dose mice, and the number of the hair follicles recovered at the same time. The result of mouse whole-genome microarray showed that there were 2 219 gene transcription changes in the drug-treated group compared with the model group, and these differential genes regulated the four mechanisms of the seven vitiligo pathogenesis reported in the literature respectively. Preliminary confirmed Quban film mechanism of action and inhibition of autoimmune hyperthyroidism, promote keratinocyte formation, reduce oxidative stress response and promote the formation of melanin. CONCLUSION: This study confirms the anti-vitiligo effect of Qubai Tablets and explores its molecular mechanism at the same time, providing the direction for further study in the later stage.